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|Molecular Diagnostic Testing|
Mystic Aquarium offers molecular diagnostic testing for both routine analysis and research projects.
For further information or to submit samples please contact:
Laura Thompson, PhD
55 Coogan Blvd.
Phone: 860-572-5955 ext. 155
Brucella sp. Real-time PCRProcedure: Real-time polymerase chain reaction (qPCR) analysis can be performed on DNA extracted from tissues, blood or fluids. This assay uses primers, probe and adapted protocols that target the gene for a 31 kDa outer membrane protein bcsp31, which is specific to genus Brucella (Probert et. al, 2004. Journal of Clinical Microbiology 42(3):1290.) The assay also includes two internal controls to assess DNA quality and presence of PCR inhibitors. Samples accepted: tissues (spleen, pulmonary and inguinal lymph nodes, tonsil, lung, liver, kidney, brain, gonad, uterus or epididymis), whole blood or buffy coat, other body fluids Specimen: small tissue samples (0.5 cm3) are required, unless simultaneous culture is desired (>2.5 cm3); >200 µl EDTA-preserved (not heparin) whole blood/buffy coat or other body fluids Sample storage: Short-term storage at -20°C is permissible, however if long term storage is necessary, tissues should be frozen at -70°C. Blood and fluids are ideally submitted fresh/cooled, but may be frozen. Sample shipment: overnight on ice packs (for fresh/blood samples) or frozen, preferably on dry ice Test days: when sufficient numbers of samples are received Minimum Test time: 5 days
|The mission of Sea Research Foundation, Inc., which includes Mystic Aquarium, Ocean Exploration Center and JASON Learning, is to inspire people to care for and protect our ocean planet through conservation, education, and research.|
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55 Coogan Blvd., Mystic, CT 06355-1997 | firstname.lastname@example.org
P: 860.572.5955 | F: 860.572.5969